This study aimed to further test the psychometric properties of the Psychological Strain Scales (PSS) with larger samples. A cluster sampling method was employed to recruit 13,250 college students from seven provinces in China. Descriptive statistics, item statistics, Cronbach’s alpha, factor analysis, and correlation analysis were evaluated. Cronbach’s alpha coefficient was 0.958 and Guttman’s split-half coefficient was 0.866. All the item-total correlations were larger than 0.45 except one. Results from Exploratory Factor Analysis and Confirmatory Factor Analysis supported the four dimensions of psychological strains (value strain, aspiration strain, deprivation strain, and coping strain). The PSS was strongly correlated to the Depression, Anxiety, and Stress Scale (DASS-21), which was commonly used to measure risk for suicide and was predictive of suicidality as measured by the SBQ-R. The PSS was a self-report instrument with high reliability and validity which could be applied for extensive use in research to measure psychological strains.